| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.032836 |
| Chromosome: | chromosome 10 |
| Location: | 2459943 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435600 | TMEM17 | Transmembrane protein 17; (1 of 1) K19384 - transmembrane protein 17 (TMEM17) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAGGTAGACAGTTGCAGCACCGCTGCCGCTCGTGCTTCACTGTGCC |
| Internal bar code: | AGTCTAACGCTCGGTAATCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1465 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGACAAGCAGCCAGAC |
| Suggested primer 2: | GGGCGGTCTTCATCTTCCAA |