| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032846 |
| Chromosome: | chromosome 7 |
| Location: | 5449218 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350867 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCAGCAGCGGGCGCCTGCTGCCGCTCCTTCTGCTATGCTGCAGCGCA |
| Internal bar code: | GGAACTAGGTGCTTCAAACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACTGCATGTAATGGCGC |
| Suggested primer 2: | GAGATCATCAGCCTGGTCGG |