| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032848 |
| Chromosome: | chromosome 12 |
| Location: | 5211901 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527150 | (1 of 1) PF09234 - Domain of unknown function (DUF1963) (DUF1963) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGGTGTATGAAGTGTACGGGAGGTTGGCACTGAGGGGAAGAAAGCCT |
| Internal bar code: | GCTGTGGCGTGTTTATTATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5177 |
| LEAP-Seq percent confirming: | 74.7368 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGACCTCACACCTTTCA |
| Suggested primer 2: | ATGACTCGGTCAAAGCCCAG |