Insertion junction: CLIP2.032854_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.032854
Chromosome:chromosome 4
Location:2146069
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219900 PGPP1 (1 of 1) K01094 - phosphatidylglycerophosphatase GEP4 (GEP4); Putative phosphatidylglycerolphosphate phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAAGGGCCTACGCGCCGGAGCTGGCCGCCAGACTGGACGGGCATCGGG
Internal bar code:TAACTGTTCGAAATTAGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:718
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGAACGTGTCTGAGGGGAAG
Suggested primer 2:TTGACGTATGCTGCCAAGGT