| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.032855 |
| Chromosome: | chromosome 2 |
| Location: | 2345166 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090700 | MAPKKK8 | Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGAATCGCAAAAACACACACGATCAGCTCCTTGCCACGCACCATCCAT |
| Internal bar code: | TATGGAGGGTCCTTGTTATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAAACCACCGGTACCGT |
| Suggested primer 2: | TTGGGGTTTGCTACGGGTTT |