| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.032856 |
| Chromosome: | chromosome 2 |
| Location: | 6971994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389356 | (1 of 1) K03457 - nucleobase:cation symporter-1, NCS1 family (TC.NCS1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGGTGTCATCCACACACGGCTTCGTCAACACCTGGCTCATCGGTGG |
| Internal bar code: | AAGCGCGCACAATATGCTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 94.5455 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGACTTGTCGCCCTTG |
| Suggested primer 2: | ATCCTGGGATGGTATGGGCT |