| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.032875 |
| Chromosome: | chromosome 1 |
| Location: | 3340285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021600 | HEL1 | (1 of 1) PTHR24031//PTHR24031:SF255//PTHR24031:SF274 - RNA HELICASE // DEAD-BOX ATP-DEPENDENT RNA HELICASE 40 // SUBFAMILY NOT NAMED; DEAD-box RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTAGGGGCGGCGTTGTCAGTTGTCAGCCAGCAGATTGGCCGGATGGT |
| Internal bar code: | CGCGATGCGTGACAGGCGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1164 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGGAGTCAGATACGGTG |
| Suggested primer 2: | CATCCTGGATGAGGTACGCC |