| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.032881 |
| Chromosome: | chromosome 3 |
| Location: | 1121516 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148850 | (1 of 1) 2.7.11.1//2.7.12.2 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Mitogen-activated protein kinase kinase / MKK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATCGCTGCTGTGGGCCAGTAAGACCTATGGAATCTGCCATGTTCGC |
| Internal bar code: | TTTGAATGGTTTCTTCTTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2345 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTGTGATGGCGAACTT |
| Suggested primer 2: | TCCACTGTCGCAAGTCTCAC |