| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032885 |
| Chromosome: | chromosome 12 |
| Location: | 7827890 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553850 | TUL1 | (1 of 781) IPR000104 - Antifreeze protein, type I; Tubulin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCAGGAGCCTCAGAAGACAGAGCCGCGCCGTCGCCGCCGGCTGTAGC |
| Internal bar code: | TGACTAGAGCAACTGACGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2228 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAGACATGGTATCGG |
| Suggested primer 2: | CGGCAGGATGGATACCGTAC |