Insertion junction: CLIP2.032894_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.032894
Chromosome:chromosome 1
Location:6681886
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g047650 (1 of 1) K10268 - F-box and leucine-rich repeat protein 2/20 (FBXL2_20) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCCAGCGCCACGTCCTCCAGGCCGCTGCAGCCCAGCGCCAGCGCCGC
Internal bar code:ATCCCGCTCTCAATTTATAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1726
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGTCCTGCTTGCTTCTGA
Suggested primer 2:CCCTTCGCTATGACTCGACC