Insertion junction: CLIP2.032903_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032903
Chromosome:chromosome 10
Location:25529
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417500 CYN20E,CYN20-5,CYN9 (1 of 1) PF00160//PF04969 - Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD (Pro_isomerase) // CS domain (CS); Cyclophilin 20-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAACTTCACGGTTGGGAGAAGGCCATGGTAAGAACTAGACAGGGGCAG
Internal bar code:TTATGTCAAAGCGGGAATCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4655
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCTCCACCACTAGCT
Suggested primer 2:GCAGTCTGTCTTGGGACCTC