| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032919 |
| Chromosome: | chromosome 9 |
| Location: | 2109438 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396050 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACGCCGGGCCCCGCGAAGACCCCATATCGTCCGGACCCTCCTATGCC |
| Internal bar code: | TTTGGTTTATTGGGAGTCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4497 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCACTTCAACAGCGGCA |
| Suggested primer 2: | CCTTGTTGACGTCCTCCTCC |