| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.032927 |
| Chromosome: | chromosome 2 |
| Location: | 5239480 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGTGGGATGCATGGCCAGGACCAAATGGTTGATTGGGGCAGTGGAGA |
| Internal bar code: | TGGGTATATGTGCTGTAGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCCAGAAGTTCCGCTG |
| Suggested primer 2: | TTGCCAAGGTTTCGGATCGA |