Insertion junction: CLIP2.032934_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.032934
Chromosome:chromosome 16
Location:3865060
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688901 CYG56 (1 of 1) PTHR11920//PTHR11920:SF345 - ADENYLATE AND GUANYLATE CYCLASES // SOLUBLE GUANYLATE CYCLASE 88E; NO-dependent guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGGACGAGCAGACCTACAAGTGAGTAGCGGCAGCACCGCATGACGCC
Internal bar code:CGGCCGTCGTATTAAACGACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1282
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGATGCCAGTCTGAGCC
Suggested primer 2:TCGCGCTATGAAGAATCGCT