Insertion junction: CLIP2.032943_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.032943
Chromosome:chromosome 12
Location:3961479
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515500 (1 of 1) PF04780 - Protein of unknown function (DUF629) (DUF629) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCAGCGTGGACGACCTGCAGCACAAGTTTGAGACGCAGGCGGCGGTGA
Internal bar code:ACTATTGCCCCACGCTGATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1381
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTCCACGTCCATGTACCG
Suggested primer 2:GACAACATCGTGGAGTGGGT