| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.032949 |
| Chromosome: | chromosome 6 |
| Location: | 1950283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264750 | HTA14 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCAGCAGAGCCGTCCTCAGCCTTGCCGCCCTTGGTCTTCTTGGGCAG |
| Internal bar code: | CCAACGGGGTAAATAAATCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3512 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCCAACACAATGGCTGG |
| Suggested primer 2: | CCTCCCACGCTTTGTTTGTG |