| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032964 |
| Chromosome: | chromosome 16 |
| Location: | 6768377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672450 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS | |
| lncRNA_TCONS_00150411 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAGCACTACCTCAGCCACAAAATCCTAAATGGGGTCTTCGAGCCTGG |
| Internal bar code: | TGTCTACTTAAATAGTCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3833 |
| LEAP-Seq percent confirming: | 38.8889 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTACCTTCTGCTGCGT |
| Suggested primer 2: | ACCCATACGCAAACCTCCAG |