| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.032964 |
| Chromosome: | chromosome 16 |
| Location: | 6768378 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672450 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS | |
| lncRNA_TCONS_00150411 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAAGTGGTGCAGTCCGCGTCGATGGAGAACGGAACTGCGAGGGCACG |
| Internal bar code: | TGTCTACTTAAATAGTCGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 73.5294 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATACGCAAACCTCCAG |
| Suggested primer 2: | AAGCTTACCTTCTGCTGCGT |