| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032992 |
| Chromosome: | chromosome 6 |
| Location: | 1758386 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263000 | (1 of 18) PF12937 - F-box-like (F-box-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGCATTAAGGCATAGCCACATCGGGTCGTGCTGGGGTGGGTTTGCAG |
| Internal bar code: | ATAAATAGGTGCTAGAAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2707 |
| LEAP-Seq percent confirming: | 81.5603 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATTGGCGTGGCAGTTGTT |
| Suggested primer 2: | ACTCCTCTCCCTCCATCACC |