Insertion junction: CLIP2.033003_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033003
Chromosome:chromosome 14
Location:240410
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g609202 Conserved Protein With Tyrosinase Domain; (1 of 1) 3.4.24.7 - Interstitial collagenase / Vertebrate collagenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGAGGCGCCCACGCTGGCACGGTTCGTGGATGTGGATCCCGTCAGGT
Internal bar code:GATCCACGGTTGATCACCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:4.34783
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCACAAACACCCATCAG
Suggested primer 2:CGGAGTTGAGGAGGAGAGGA