Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.033013 |
Chromosome: | chromosome 6 |
Location: | 4317584 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278263 | (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGCGTGAGCGCCAGGGTGGCGAGTGGGAGAGCACTTACAGGCTGTA |
Internal bar code: | GTCTAGAGCTTGGTGAAATACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2067 |
LEAP-Seq percent confirming: | 70.5882 |
LEAP-Seq n confirming: | 12 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCCGCTCGCTGTATTGAT |
Suggested primer 2: | CTGGGGGAGGAAGCAAAGAG |