| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033016 |
| Chromosome: | chromosome 10 |
| Location: | 237533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419300 | CDS | ||
| Cre10.g419350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAACAATGTGCAACCCTTGGCTGTTGACCCAGCAACTCTCCTTGACA |
| Internal bar code: | GGGACGCGGATCCGTACGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 67.6471 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACCTTACCACTCGCCCC |
| Suggested primer 2: | TCAACTTGGCCTTTTGGGGT |