| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033017 |
| Chromosome: | chromosome 8 |
| Location: | 4426992 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385400 | (1 of 3) 3.5.1.1 - Asparaginase / L-asparagine amidohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCTGCCCCTGCTCCTGCCCTGGCGCCTGGTCCGGGCGGTGCAGCTG |
| Internal bar code: | AAACCGAATGTCAGGTAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1496 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGTTTGCTCGCCGGATT |
| Suggested primer 2: | CTCTGTGAGCTGGTTCTGGG |