| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.033028 |
| Chromosome: | chromosome 10 |
| Location: | 199629 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419050 | ATP1B | Mitochondrial F1F0 ATP synthase, alpha subunit; (1 of 2) PTHR15184:SF3 - ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTGGACCGCAGGAGCACTGCGGCAGGTGGGACAGCAGGTGGGTCAG |
| Internal bar code: | TTTGCACAGCAAAAATATAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAGCAGTAGTAGGGTCA |
| Suggested primer 2: | CTGCGGAGTTTGGGAAGGAT |