| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.033049 |
| Chromosome: | chromosome 15 |
| Location: | 818549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639056 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGTGCCGGCGACATGTGAAGAATGCGGGAGAGCAAAGGACAGCGAT |
| Internal bar code: | GTGAGGGTATCAAGTCGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1930 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACAACCTCGTGACAC |
| Suggested primer 2: | TGAGGGATGAGGGAGTGAGG |