| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033050 |
| Chromosome: | chromosome 10 |
| Location: | 4224917 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449350 | (1 of 1) PTHR11717//PTHR11717:SF2 - LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE // PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCTGTCCAAGGCGCCCATTGGATAGTGCTGAGCTGTTGTGGTGCCG |
| Internal bar code: | CGGGTCTTCTGGCAGCGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5230 |
| LEAP-Seq percent confirming: | 90.7975 |
| LEAP-Seq n confirming: | 148 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 163 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTCGTTTGGCTGTCCA |
| Suggested primer 2: | GGCTCAGTCGTATCCAGACG |