| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033056 |
| Chromosome: | chromosome 6 |
| Location: | 8670861 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310000 | AP4E1 | (1 of 1) K12400 - AP-4 complex subunit epsilon-1 (AP4E1); Epsilon4-Adaptin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACGGCATGTACGGTACTACGACCTGCATGTGTG |
| Internal bar code: | GCATTGGAGGGGATAGATCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2035 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCAAGGAGCATGGGGAA |
| Suggested primer 2: | CAAAGAGAGAGGCAGCGAGT |