Insertion junction: CLIP2.033067_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.033067
Chromosome:chromosome 9
Location:1319650
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400550 NOA1 Nitric oxide associated protein; (1 of 1) K13427 - nitric-oxide synthase, plant (NOA1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCCCCAAATGCCCCAAACCCCCACCCTCCACGCCGCCTCCACTACCA
Internal bar code:AACGCGCACGCTGATCATAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2620
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCGTCTGCATTTCTGTGA
Suggested primer 2:GACACAGCCCACACTCTACC