| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033068 |
| Chromosome: | chromosome 16 |
| Location: | 6509301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674650 | (1 of 1) 3.5.1.59 - N-carbamoylsarcosine amidase / N-carbamoylsarcosine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATCGCGCCAAAGTATTGCACAGTACTCTTACACAGGCGCTGGGGCTG |
| Internal bar code: | CGTTGTAGTGTGGGTCTGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1659 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGTTCGAAGTCGTCACA |
| Suggested primer 2: | AGGGGAAATGTCCTGGCATG |