| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033089 |
| Chromosome: | chromosome 2 |
| Location: | 226107 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800153 | intron | ||
| Cre02.g800155 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGAGCATGGAGCTAATAGACCTGCAGCTGCTGCGGCGCAATGGGGACT |
| Internal bar code: | TCAGTGAGATATCGGATAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3607 |
| LEAP-Seq percent confirming: | 55.1181 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCAATCAACAGCAACAA |
| Suggested primer 2: | CCTCCCTCGTGTTCTGGTTC |