| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.033094 |
| Chromosome: | chromosome 12 |
| Location: | 5996430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534300 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCCTGCTGCGGCGGTGGTGGATGGCAGCGGTGGAGGTGGAGGCGGA |
| Internal bar code: | GCTCAGGGTAGTATCTCAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3198 |
| LEAP-Seq percent confirming: | 84.0909 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCTGCTGCTACTGTT |
| Suggested primer 2: | ACGTGGTGCCTGTAACTCAG |