| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033101 |
| Chromosome: | chromosome 10 |
| Location: | 2918493 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439900 | HSP70G | ER-located HSP110/SSE-like protein; (1 of 1) K09486 - hypoxia up-regulated 1 (HYOU1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCTTGGGCTTGCCCGCCTCCTTGACCGTGTAGACTGAGAACTTGACC |
| Internal bar code: | GCGCCGTTAGGGGGCCAAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1363 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATGTGCTTTCCTACCG |
| Suggested primer 2: | GGGGGCGTACTTCATTCCAA |