| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.033106 |
| Chromosome: | chromosome 9 |
| Location: | 1877015 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTCGCACGGGCTGAGTCAGTTCCAGATCCAGATGGCGGCGTCGCTGC |
| Internal bar code: | TGGGGCGGGGGACGCCCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1808 |
| LEAP-Seq percent confirming: | 95.3125 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAAACGCTGGGTTATGG |
| Suggested primer 2: | GTCTCCTCCCTGACCCTGAT |