| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033113 |
| Chromosome: | chromosome 7 |
| Location: | 1387281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322900 | (1 of 1) K13806 - sn1-specific diacylglycerol lipase (DAGL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATATGGAGCCTGCGGCGTGAATGGTATACAGCGGTTGGTGGTTCAGAT |
| Internal bar code: | ATCATCATTTCGAATGAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4679 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCTTGCCTGCCAGGTG |
| Suggested primer 2: | ATGGCCCATCTCCTACCTGT |