| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033114 |
| Chromosome: | chromosome 2 |
| Location: | 1859453 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087300 | HPR3 | Hydroxypyruvate reductase 3; (1 of 1) 1.1.1.81 - Hydroxypyruvate reductase / D-glycerate dehydrogenase | intron |
| Cre02.g800184 | (1 of 7) IPR010995 - DNA repair Rad51/transcription factor NusA, alpha-helical | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCTCGGCCTGTGCGTGGGCATGTCTTAGCTCTTATCCAGCGCGGCCG |
| Internal bar code: | AAACAGTTTCGTAACTATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4723 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACACTCGCCACGCATAT |
| Suggested primer 2: | CAACCAAAGCCACCACATCG |