| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033116 |
| Chromosome: | chromosome 14 |
| Location: | 199889 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801598 | (1 of 3) 3.5.5.1 - Nitrilase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGACACGGGGGTGCTCGGCTCATGGAAAGGCGTGGAACGGGTTGTGC |
| Internal bar code: | CGGCATCGATTATCTCAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 62.963 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGGTATTCGCAACCCCA |
| Suggested primer 2: | GCGTGCGTGTGTGTATGATC |