| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033121 |
| Chromosome: | chromosome 9 |
| Location: | 6528255 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415800 | (1 of 1) K16865 - programmed cell death protein 4 (PDCD4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTGCCACCTCCGCTGAGTCCTGTGAGTCGAAGTACTCCCGCACAGCC |
| Internal bar code: | AGCCTGTATAGTAACGGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4416 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGGTTCGGCATTGAGG |
| Suggested primer 2: | TTGTCCTCGTGTGAGACTGC |