Insertion junction: CLIP2.033148_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.033148
Chromosome:chromosome 8
Location:875686
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361450 (1 of 1) IPR008867//IPR009836 - Thiazole biosynthesis // Protein of unknown function DUF1399 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCGCCGGCAGTGTCCACGCCGCCACCCGCGTCCCACAGCCCGCCAGT
Internal bar code:CAGATTAGGACAGCAAATTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:859
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCACGTCAATGAGTCCG
Suggested primer 2:GCGGCTCAACATCCATGAAC