| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.033155 |
| Chromosome: | chromosome 3 |
| Location: | 5211997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181750 | intron | ||
| lncRNA_TCONS_00234913 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGGAACAGGTCAGCAGGTGCAGGCAGACAGCGCCCCGCCAGCTGGC |
| Internal bar code: | TGCGCACCCATCCTAATGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3547 |
| LEAP-Seq percent confirming: | 32.8358 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGCTCCGAGTACTTGT |
| Suggested primer 2: | AAGGAGGGAGGCTAGGGAAG |