Insertion junction: CLIP2.033182_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033182
Chromosome:chromosome 1
Location:1807606
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010100 NAT7 N-acetyltransferase; (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTTTACATCAGATGTAGGCAAGGATTAGCAGGGTGTGCGTAATCTGC
Internal bar code:GGGATCCGAGACAGTATGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2439
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCATGCACCAACGAATGC
Suggested primer 2:CTGCCACCAGCTTCTCAAGA