Insertion junction: CLIP2.033182_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.033182
Chromosome:chromosome 1
Location:1807611
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010100 NAT7 N-acetyltransferase; (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCACAGCTTGGAAATGACTTCCACTGGTCCACTGCACGGTGCTGCAG
Internal bar code:TCTTCATTGGGCCCGCTTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:899
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCACCAGCTTCTCAAGA
Suggested primer 2:CTCCATGCACCAACGAATGC