Insertion junction: CLIP2.033182_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033182
Chromosome:chromosome 2
Location:4141926
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800224 CDS
lncRNA_TCONS_00041505 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGCGCACATGAGCGGTATCGCCTGTGTCGTGTACTCGTTCGGCTCGG
Internal bar code:TCTTGTTATTTGCGATGATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:4377
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTGGGGCATCTAGATGT
Suggested primer 2:ATTGGGGGCTATGGGTTGTG