Insertion junction: CLIP2.033186_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.033186
Chromosome:chromosome 12
Location:2467759
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g507450 SYP4 Trans-Golgi network Qa-SNARE protein; (1 of 1) K08489 - syntaxin 16 (STX16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCACGCTGGGGCATGTGGGGCGCGTGTACCGCGGGCCGTGGCGAGCA
Internal bar code:CATGTCTCCGTGGTTGAAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:229
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGCCTCCACGATCTTGCG
Suggested primer 2:GTGAAGGAGCGACTGGTGAA