| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.033188 |
| Chromosome: | chromosome 12 |
| Location: | 7732474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554700 | SEC22 | Qb-SNARE protein, Sec20-family; (1 of 1) K08517 - vesicle transport protein SEC22 (SEC22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACGCCTCTCCGCGACAAGGAGGGCCTGGCTTAAATGCAATTGATATT |
| Internal bar code: | GTGATGGAATTAAATTATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGACTGGGTAGGACCGG |
| Suggested primer 2: | GCCAGCTGCAGATACCGTAT |