| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033203 |
| Chromosome: | chromosome 11 |
| Location: | 3118974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475450 | (1 of 1) K11863 - Ataxin-3 (ATXN3, MJD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGTGTTATCCCCCGCCTAGCCTTTGCTGCCCTGATGTTTGGCCAGCT |
| Internal bar code: | TCCCTTGGCCATGGCAACGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5646 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGCAGATGAAGGCCTC |
| Suggested primer 2: | CGCGGCATTCTCATTCTTGG |