| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033217 |
| Chromosome: | chromosome 13 |
| Location: | 992258 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568350 | BLZ18 | bZIP transcription factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCGAGGACCCTCCGTCAGTCAGCTTGCAAGAACGTGCCCTGTGTGT |
| Internal bar code: | GTCCTGGGTTAGCCAGCTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAAACCTCCTGGCTAT |
| Suggested primer 2: | CATGAGGCTTGCAAAAGCGT |