| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033221 |
| Chromosome: | chromosome 3 |
| Location: | 1730598 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800300 | (1 of 73) IPR000477//IPR005135 - Reverse transcriptase domain // Endonuclease/exonuclease/phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGCGCGTGTCATTCACGCGATAAGGCTGCGACGTAACGACCTTACG |
| Internal bar code: | GCTCTAATCTTCCAGCTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2855 |
| LEAP-Seq percent confirming: | 56.4516 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCGACACTACCTCGGAG |
| Suggested primer 2: | ATCGAGCACTAGAGCATGGC |