| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033222 |
| Chromosome: | chromosome 12 |
| Location: | 8796198 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546950 | 3'UTR | ||
| Cre12.g547001 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCAACATGCACGCCGTTGCCGTGCTCTCCTCCTCCACGTGGCCTTGC |
| Internal bar code: | AATCGAGCTACGGGCTACTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4483 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGAAGGCCACGACAAG |
| Suggested primer 2: | TGCTCATATGGAATCGGCCC |