| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.033225 |
| Chromosome: | chromosome 3 |
| Location: | 8859793 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205809 | (1 of 1) PF01367//PF02739 - 5'-3' exonuclease, C-terminal SAM fold (5_3_exonuc) // 5'-3' exonuclease, N-terminal resolvase-like domain (5_3_exonuc_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCTCATCCTTGGGCGTACGCCCCGCCTTGTATGCCTGAGGCGGCAGC |
| Internal bar code: | TGTGTTTGCTCTGGCCCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGTGTTGCCACCAGGA |
| Suggested primer 2: | GCTCGTCAAAGGGGAAGTCA |