Insertion junction: CLIP2.033230_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033230
Chromosome:chromosome 3
Location:7667213
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g204050 ELG6 Exostosin-like glycosyltransferase 6; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase 5'UTR
Cre03.g204100 ELG5 Exostosin-like glycosyltransferase 5; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGCTCTATGCATCATTGATGAACTCGTCATCCAAATCCACTTTCACAG
Internal bar code:GCATGCCACGTACGAATACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3143
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCCACAGGTACCGCTAT
Suggested primer 2:TCGTCCGCGATGGTAATCTG